2110 pFastBac-Dual-VSVG-HIVGag-FLPe-CMV-Flpe

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Cloning of 2110 pFastBac-Dual-VSVG-HIVGag-FLPe-CMV-Flpe

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Purpose: Cloning of 2110 pFastBac-Dual-VSVG-HIVGag-FLPe-CMV-Flpe

Date: 2014-6

Notebook:0

Page:

Clone ID:2110

Experimenters: shanyi

Results

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subcloning

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subclone CMV-Flpe from 471 pAAV.CMV-FLPe using Not1 + Xba1 to 2226

471digest with Not1 + Xba1+BsaI,CUT the first band



Colony Screening

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Date: 2014-6

mini prep and digest with EcoR1


verify using Not1 + Xba1



Result intepretation/Summary

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  • <br>

Conclusion

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<br>