2114 pEC-iFLP
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Cloning of 2114 pEC-iFLP
[edit]Purpose: Cloning of 2114 pEC-iFLP==
Date: 2014-2----
Notebook:0
Page:
Clone ID:2114
Experimenters: shanyi
Results
[edit]CLONING
[edit]Gene synthesis of 2 fragments,
Join by overlapping PCR
Cut kfu pcr with HindIII + Xho1,
Clone into HindIII + Xho1 digested vector
Colony Screening
[edit]Date: 2014
Make miniprep, digest with HindIII + Xho1
