2115 2108 1142 1992

06/17/2014

Lane	Name	Protein Name	Clone ID	Host	Induction	Purification	sample
1	2115 pT7A3-iFLP pI/Mw: 9.76 / 52.8	iFLP	2115	15 ml, Tuner(DE3)-pG-KJE8	50uM IPTG  20oC o/n	Nickel Magnetic Bead	S
2				1 5 ml, Tuner(DE3)-pGro7	50uM IPTG 20oC o/n	Nickel Magnetic Bead	S
3				15 ml, Tuner(DE3)-pKJE7	50uM IPTG 20oC o/n	Nickel Magnetic Bead	S
4				15 ml, Tuner(DE3)-pGTf2	50uM IPTG 20oC o/n	Nickel Magnetic Bead	S
5				15 ml, Tuner(DE3)-pTf16	50uM IPTG 20oC o/n	Nickel Magnetic Bead	S
6				Nico21DE3plysN	50uM IPTG 20oC o/n	Nickel Magnetic Bead
7				TunerDE3plysN	50uM IPTG 20oC o/n	Nickel Magnetic Bead	s
8	pCold-Tat-NLS-iFLP-6H pT7A3-iFLP 9.76 / 52.8	iFLP	2108	TunerDE3plysN	50uM IPTG 20oC o/n	Nickel Magnetic Bead	s
	PageRuler Plus Prestained protein ladder
9	pLN6H-Tth-SSB    5.7/31.1		1142	Nico21DE3plysN	grow at 30oC, induce at 42oC o/n	Nickel Magnetic Bead	s
10	pL42-SM2 6.54/27.9		1992	Nico21DE3plysN	grow at 30oC, induce at 42oC o/n	Nickel Magnetic Bead	s
11	MBP-Taq-Muts		211		Eluted from NI column

Lane	Name	Protein Name	Clone ID	Host	Induction	Purification	sample
2	2115 pT7A3-iFLP pI/Mw: 9.76 / 52.8	iFLP	2115	15 ml, Tuner(DE3)-pG-KJE8	50uM IPTG  20oC o/n	Nickel  Bead	p
3				1 5 ml, Tuner(DE3)-pGro7	50uM IPTG 20oC o/n	Nickel  Bead	p
4				15 ml, Tuner(DE3)-pKJE7	50uM IPTG 20oC o/n	Nickel Bead	p
5				15 ml, Tuner(DE3)-pGTf2	50uM IPTG 20oC o/n	Nickel  Bead	p
6				15 ml, Tuner(DE3)-pTf16	50uM IPTG 20oC o/n	Nickel  Bead	p
7				Nico21DE3plysN	50uM IPTG 20oC o/n	Nickel Bead
8				TunerDE3plysN	50uM IPTG 20oC o/n	Nickel  Bead	p
9	pCold-Tat-NLS-iFLP-6H pT7A3-iFLP 9.76 / 52.8	iFLP	2108	TunerDE3plysN	50uM IPTG 20oC o/n	Nickel  Bead	p
	PageRuler Plus Prestained protein ladder
10	pLN6H-Tth-SSB    5.7/31.1		1142	Nico21DE3plysN	grow at 30oC, induce at 42oC o/n	Nickel  Bead	p
11	pL42-SM2 6.54/27.9		1992	Nico21DE3plysN	grow at 30oC, induce at 42oC o/n	Nickel Bead	p
12