2125-pCMV5-tat-CD63-TagGFP
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Cloning of 2125-pCMV5-tat-CD63-TagGFP
[edit]Purpose: Cloning of 2125-pCMV5-tat-CD63-TagGFP
Date: 2014-3-18---2014-3-27
Notebook:0
Page:
Clone ID:2125
Experimenters: yingying
Results
[edit]cloning
[edit]
PCR CD63 from 2120 pJP1520-CD63
fragment 1
CD63-BamH1-F
CD63-tat-R
fragment 2
CD63-tat-F
CD63-EcoR1-R
no PCR product: 2125pcr1(450bp),
2126pcr1(450bp),
2121PCR(730bp)
2014-3-19
| 1 |
2 |
3 |
4 |
5 |
6 |
7 |
8 |
9 |
10 |
11 |
12 |
13 |
14 |
15 |
||
| 2124T |
2128pcr1 |
2128pcr2 |
2128pcr3 |
2125pcr1 |
2125pcr2 |
2126pcr1 |
2126pcr2 |
2121PCR |
2127pcr1 |
|||||||
| product |
yes |
yes |
yes |
yes |
no |
yes |
no |
yes |
no |
yes |
no PCR product: 2125pcr1(450bp),
2126pcr1(450bp),
2121PCR(730bp)
PCR CD63 from 2153 pDONR221-CD63
fragment 1
CD63-BamH1-F
CD63-tat-R
fragment 2
CD63-tat-F
CD63-EcoR1-R
pcr1+pcr2
| lane |
7 | 8 |
| PCR | 1888 pCMV5-Gag-TagGFP-WPRE-oriP | |
| BamH1 + EcoR1 | ||
Colony Screening
[edit]Date: 204-3-27
Make miniprep and digest with BamH1 + EcoR1
