2126-pCMV5-pene-CD63-TagGFP

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Cloning of 2126-pCMV5-pene-CD63-TagGFP

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Purpose: Cloning of 2126-pCMV5-pene-CD63-TagGFP

Date: 2014-3-20---2014-3-27

Notebook:0

Page:

Clone ID:2126

Experimenters: yingying

Results

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cloning

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PCR CD63 from 2120 pJP1520-CD63

pcr1
CD63-BamH1-F
CD63-pene-R

pcr 2
CD63-pene-F
CD63-EcoR1-R


PCR :pcr1+pcr2


lane
 9
 10

 PCR
 1888 pCMV5-Gag-TagGFP-WPRE-oriP

 BamH1 + EcoR1


Colony Screening

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Date: 2014-3-27

Make miniprep and digest with BamH1 + EcoR1


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Result intepretation/Summary

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Conclusion

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