2127-pCMV5-Mfge8-Tat-TagGFP-PDGFR
Jump to navigation
Jump to search
Cloning of 2127-pCMV5-Mfge8-Tat-TagGFP-PDGFR
[edit]Purpose: Cloning of 2127-pCMV5-Mfge8-Tat-TagGFP-PDGFR
Date: 2014-3-19---2014-3-24
Notebook:0
Page:
Clone ID:2127
Experimenters: yingying
Results
[edit]cloning
[edit]
1. PCR MFGE8 ss from 2124 pLX304-MFGE8 using
Mfge8-BamH1-F
Mfge8-Nhe1-6XHis-R
2. PCR 6XHis-Tat-NLS from 1981 pCMV5-Tat-Flp-PDGFR using
Mfge8-Nhe1-6Xhis-F
NLS-TagGFP-R
3. PCR TagGFP from clone1970 TaqGFP using
NLS-TagGFP-F
TagGFP-nostop-Kpn1-R
pcr1+pcr2+pcr3
| lane |
5 |
6 |
| PCR |
1981 pCMV5-Tat-Flp-PDGFR | |
| BamH1 + Kpn1 | ||
Colony Screening
[edit]Date: 2014-3-24
Make miniprep and digest with BamH1 + Kpn1
c:1981 pCMV5-Tat-Flp-PDGFR
<br>
Result intepretation/Summary
[edit]Conclusion
[edit]