2128-pCMV5-Mfge8-Tat-TagGFP-MFGE8
Cloning of 2128-pCMV5-Mfge8-Tat-TagGFP-MFGE8
[edit]Purpose: Cloning of 2128-pCMV5-Mfge8-Tat-TagGFP-MFGE8
Date: 2014-3-20---2014-3-28
Notebook:0
Page:
Clone ID:2128
Experimenters: yingying
Results
[edit]cloning
[edit]
| 3 |
4 |
5 | |
| pcr1 |
pcr2 |
pcr3 | |
pcr1: gene synthesis
MFGE8-1 to MFGE8-4
pcr2:2124 pLX304-MFGE8 as template
MFGE8-Kpn1-F
MFGE8-R
pcr3:2124 pLX304-MFGE8 as template
MFGE8-F
MFGE8-Not1-R
pcr1+pcr2+pcr3
MFGE8-Kpn1-F+MFGE8-Not1-R
| lane |
3 |
4 |
| PCR |
2127 pCMV5-Mfge8-Tat-TagGFP-PDGFR | |
| Kpn1 + Not1 | ||
Colony Screening
[edit]Date: 2014-3-28
Make miniprep and digest with Kpn1 + Not1
<br>
Result intepretation/Summary
[edit]Conclusion
[edit]
Cloning of 2128-pCMV5-Mfge8-Tat-TagGFP-MFGE8
[edit]Purpose: Cloning of 2128-pCMV5-Mfge8-Tat-TagGFP-MFGE8
Date: 2014-3-31---2014-4-3
Notebook:0
Page:
Clone ID:2128
Experimenters: yingying
Results
[edit]cloning
[edit]
| 3 |
4 |
5 | |
| pcr1 |
pcr2 |
pcr3 | |
pcr1: gene synthesis
MFGE8-1 to MFGE8-4
pcr2:2124 pLX304-MFGE8 as template
MFGE8-Kpn1-F
MFGE8-R
pcr3:2124 pLX304-MFGE8 as template
MFGE8-F
MFGE8-Not1-R
pcr1+pcr2+pcr3
MFGE8-Kpn1-F+MFGE8-Not1-R
| lane |
1 |
2 |
| PCR |
2127 pCMV5-Mfge8-Tat-TagGFP-PDGFR | |
| Kpn1 + Not1 | ||
Colony Screening
[edit]Date: 2014-4-3
Make miniprep and digest with Kpn1 + Eag1
<br>
Result intepretation/Summary
[edit]Conclusion
[edit]