2130-pT7A3-6H-NLS-Cre

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Cloning of 2130-pT7A3-6H-NLS-Cre

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Purpose: Cloning of 2130-pT7A3-6H-NLS-Cre

Date: 2014-3-20--2014-3-24

Notebook:0

Page:

Clone ID:2130

Experimenters: yingying

Results

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cloning

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2130 (not 2030)

PCR from Tat-Cre 298 pTriEx-HTNC
HNCre-Nco1-F
Cre-Xho1-R

Lane
7
8

PCR
1279 pT7A3

Nco1 + Xho1


Colony Screening

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Date: 2014-3-24

Make miniprep and digest with Nco1 + Xho1


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Result intepretation/Summary

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Conclusion

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