2132 pEB-T4-Endo-IV

Cloning of 2132 pEB-T4-Endo-IV =

Purpose: Cloning of2132 pEB-T4-Endo-IV==

Date: 2014-3----

Notebook:0

Page:

Clone ID:2132

Experimenters:  shanyi

Results

CLONING

 1560-5 ,missing 1 G, can be fixed by PCR using primer
T4-Endo-IV-1 TAGCACCATATGCAGAAAACGAATCCTGGGTTACAGCGTCTGTTTCAGATTCCGACCT
T4-Endo-IV-14 GGTCGTACTCGAGAATGGAAAGATACCATCCGTTGTAGTTGCTTTTGC

.digest with Nde + Xho1 clone to 2048 pEB-C6H

Colony Screening

Date: 2014_2_

Make miniprep, digestion with Nde + Xho1

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