2133 pT7A3-ILK
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Cloning of 2133 pT7A3-ILK
[edit]Purpose: Cloning of2133 pT7A3-ILK==
Date: 2014-3----
Notebook:0
Page:
Clone ID:2133
Experimenters: shanyi
Results
[edit]CLONING
[edit]PCR from Harvard Kinase HRG007900 32 H04 147913 HsCD00000155 FUSION pDNR-DUAL 3611 ILK 1359 0-1-0-0-1-0-1-0-0
ILK-Nco1-Nhe1-F tagcac ccatg ggt agc agc gct agc GACGACATTTTCACTCAGTGC
ILK-Not1-Xho1-R (CTTGAGAAGATGCAGGACAAG CAC CAt CAC CAt CAC CAt TAG CGGCCG ctcgag atagca) tgctatctcgagCGGCCGCTAaTGGTGaTGGTGaTGGTGCTTGTCCTGCATCTTCTCAAG
cut with Nco1 + Xho, clone into 1279
Colony Screening
[edit]Date: 2014_3_
Make miniprep, digestion with Nco1 + Xho
