2138 pCMV5-pene-CD63-iCre
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Cloning of 2138 pCMV5-pene-CD63-iCre
[edit]Purpose: Cloning of2138 pCMV5-pene-CD63-iCre==
Date: 2014-4----
Notebook:0
Page:
Clone ID:2138
Experimenters: shanyi
Results
[edit]CLONING
[edit]subclone pene-CD63 from 2126 pCMV5-pene-CD63-TagGFP to 1968 pCMV5-Gag-p2b-NLS-Cre-WPRE-oriP using Sal1 + EcoR1
Colony Screening
[edit]Date: 2014_4_
Make miniprep, digestion with BamH1 + EcoR1
there is one BamHI site in cre, so use BamH1 + EcoR1 to digest ,we will get there bands
