2145-pT7A3HC-6H-Tat-MS2
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Cloning of 2145-pT7A3HC-6H-Tat-MS2
[edit]Purpose: Cloning of 2145-pT7A3HC-6H-Tat-MS2
Date: 2014-4-6---2014-4-9
Notebook:0
Page:
Clone ID:2145
Experimenters: yingying
Results
[edit]cloning
[edit]
Use MS2-1 + MS2-linker-R to amplify oligo mixture of MS2-1 to MS2-12
| lane |
5 |
6 |
| PCR |
1885 pT7A3-HC | |
| Nco1 + BamH1 | ||
Colony Screening
[edit]Date: 2014-4-9
Make miniprep and digest with Nco1 + BamH1
c:vector
<br>
Result intepretation/Summary
[edit]Conclusion
[edit]