2154 pCMV5-Gag-Cre-IRES-VSVG-WPRE-oriP
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Cloning of 2154 pCMV5-Gag-Cre-IRES-VSVG-WPRE-oriP =
[edit]Purpose: Cloning of2154 pCMV5-Gag-Cre-IRES-VSVG-WPRE-oriP==
Date: 2014-3----
Notebook:0
Page:
Clone ID:2154
Experimenters: shanyi
Results
[edit]CLONING
[edit]subclone cre from 1400using EcoR1 + Not1 to 2113 pCMV5-Gag-TagGFP-IRES-VSVG-WPRE-ori
Colony Screening
[edit]Date: 2014_2_
Make miniprep, digestion with EcoR1 + Not1