2156-pJ912-HIS4
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Cloning of 2156-pJ912-HIS4
[edit]Purpose: Cloning of 2156-pJ912-HIS4
Date: 2014-4-14--2014-4-23
Notebook:0
Page:
Clone ID:2156
Experimenters: yingying
Results
[edit]cloning
[edit]
PCR HIS4 from 2158 pPIC9k
HIS4-BamH1-F
HIS4-ILV5-R
2. PCR ILV5 from 1842 pJ912-Irisin-1
HIS4-ILV5-F
ILV5-Nde1-R
pcr1+pcr2
| lane |
3 |
4 |
| pcr |
1842 pJ912-Irisin-1 | |
| BamH1 + Nde1 | ||
Colony Screening
[edit]Date: 2014-4-23
Make miniprep and digest with BamH1 + Nde1
<br>
Result intepretation/Summary
[edit]Conclusion
[edit]
Cloning of 2156-pJ912-HIS4
[edit]Purpose: Cloning of 2156-pJ912-HIS4
Date: 2014-5-1---2014-5-7
Notebook:0
Page:
Clone ID:2156
Experimenters: yingying
Results
[edit]cloning
[edit]
PCR HIS4 from 2158 pPIC9k
HIS4-BamH1-F
HIS4-ILV5-R
2. PCR ILV5 from 1842 pJ912-Irisin-1
HIS4-ILV5-F
ILV5-Nde1-R
pcr1+pcr2
| lane |
3 |
4 |
| pcr |
1842 pJ912-Irisin-1 | |
| BamH1 + Nde1 | ||
Colony Screening
[edit]Date: 2014-5-7
Make miniprep and digest with BamH1 + Nde1/Nde1+Sal1
<br>
2156-3 pcr screen:AOX1-seq-F_R+HIS4-ILV5-R
Result intepretation/Summary
[edit]Conclusion
[edit]