2189-pEC2-Tth-SOD

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Cloning of 2189-pEC2-Tth-SOD

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Purpose: Cloning of 2189-pEC2-Tth-SOD

Date: 2014-4-24---2014-4-28

Notebook:0

Page:

Clone ID:2189

Experimenters: yingying

Results

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cloning

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PCR from Thermus thermophilus HB27

Tth-SOD-Nde1-F
Tth-SOD-EcoR1-R2






PCR
2015 pEC2

Nde1 + EcoR1




Colony Screening

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Date: 2014-4-28

Make miniprep and digest with Nde1 + EcoR1


Make miniprep and digest with Xho1


clone 3 right


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Result intepretation/Summary

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Conclusion

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