2201 2213 2214 2215 2233Gateway reaction QC

2014/09 Gateway reaction and transfection/expression

small scale gateway reaction using GFP 393

2201 pCMV5-Dest-Avi-8H

2215 pCMV5-Dest-Avi-8H-BirA

2233 pCMV5-Dest-C-HA

2213 pET30b-Dest-C-Avi-His

2214 pET30b-Dest-Avi-8H-BirA

miniprep

2201, 2215,2233 gateway reaction clones digest with BamH1 + EcoR1(GFP=>790bp, DEST=>941bp)

2213,2214 gateway reaction clones digest with NcoI+KpnI(no NcoI site in 2213,2214)

transfect 2201, 2215,2233 gateway reaction clones to 293