2210-pT7A3E-ULP1

Cloning of 2210-pT7A3E-ULP1

Purpose: Cloning of 2210-pT7A3E-ULP1

Date: 2014-6-20---2014-6-26

Notebook:0

Page:

Clone ID:2210

Experimenters: LIANGHUO

Results

cloning

PCR from 2209 ZM552-ULP1
SUMO-P1-Nde1-F
tagca catatg ctt gtt cct gaa tta aat g
Sumo-Xho1-R
(gattttaaccgacgctttaaaactcgag tagcac)
gtgctactcgagttttaaagcgtcggttaaaatc
digest with Nde1 + Xho1

2210 DIGESTED

Colony Screening

Date: 2014-6-26

Make miniprep and digest with Nde1 + XhoI

SELECT CLONE5 FOR SEQUENCING

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