2213 pET30b-Dest-C-Avi-His
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Cloning of 2213 pET30b-Dest-C-Avi-His
[edit]Purpose: Cloning of2213 pET30b-Dest-C-Avi-His==
Date: 2014-8----
Notebook:0
Page:
Clone ID:2213
Experimenters: shanyi
Results
[edit]CLONING
[edit]PCR gateway cassette from 1336
1 ul of 10 uM attR1-Fix-Nde1-F tagcac cat atg gaa TCAACAAGTTTGTACAAAAAAG
1 ul of 1 uM attR2-AviTag-R2 ( ctttcttgtacaaagtggt GGCGGCCTGAACGACATCTTCGAGGCCCAGAAAATCGAGTGGCAC) GTGCCACTCGATTTTCTGGGCCTCGAAGATGTCGTTCAGGCCGCCaccactttgtacaagaaag
1 ul of 10 uM attR2-AviTag8H-Kpn1-EcoR1-R1 (CAGAAAATCGAGTGGCACGAG CAC CAC CAT CAT CAC CAT CAC CAC TGA GGTACC gaattc tacga) tcgtagaattcGGTACCTCAGTGGTGATGGTGATGATGGTGGTGCTCGTGCCACTCGATTTTCTG
digest with Nde1 + EcoR1, clone into Nde1 + EcoR1 digested vector 16 pET30b.Amp,
Colony Screening
[edit]Date: 2014_7
Make miniprep, use Nde1 + EcoR1to find positive clone
Result intepretation/Summary
[edit]Conclusion
[edit]