2216-pCMV5-BirA
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Cloning of 2216-pCMV5-BirA
[edit]Purpose: Cloning of 2216-pCMV5-BirA
Date: 2014-7-01---2014-7-02
Notebook:0
Page:
Clone ID:2216
Experimenters: LIANGHUO
Results
[edit]cloning
[edit]PCR from BL21 genomic DNA
BirA-22nt-IRES-EcoR1-Xho1-F
g taa gaattc ctcgag TTTATAATTTCTTCTTCCAGAA GCCACC ATGAAGGATAACACCGTGCCAC
BirA-Kpn1-Not1-R
(CCTGCGTAGTGCAGAAAAATAA GGTACC GCGGCCGC tagcac)
gtgctaGCGGCCGCGGTACCTTATTTTTCTGCACTACGCAGG
2216 DIGESTED
[edit]
Colony Screening
[edit]Date: 2014-7-02
Make miniprep and digest with Nde1 + XhoI
SELECT CLONE1 FOR SEQUENCING
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Result intepretation/Summary
[edit]Conclusion
[edit]