2234 pLV-Dest-C-HA

Cloning of 2234 pLV-Dest-C-HAA

Purpose: Cloning of2234 pLV-Dest-C-HA==

Date: 2014-8----

Notebook:0

Page:

Clone ID:2234

Experimenters:  shanyi

Results

CLONING

subclone ~1.9 kb CMV-Dest-HA cassette from 2233 pCMV5-Dest-C-HA using Nde1 + Nhe1, there are many Nde1 sites in the parental vector 2233 pCMV5-Dest-C-HA, so use Nde1 cut as control to find out which band should be excised from gel

Colony Screening

Date: 2014_8_

Make miniprep, use NdeI+BamH1 to find positive clone

•