2238 pT7A3-uvrD
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Cloning of 2238 pT7A3-uvrD
[edit]Purpose: Cloning of2238 pT7A3-uvrD==
Date: 2014-7----
Notebook:0
Page:
Clone ID:2238
Experimenters: shanyi
Results
[edit]CLONING
[edit]PCR from MG1655
digest with Nco1 + XhoI, clone to 1279
Colony Screening
[edit]Date: 2014_7
Make miniprep, use Nco1 + XhoI to find positive clone
use NdeI+BamHI to verify
sent 2238-1to sequence, maybe missing two nt
Result intepretation/Summary
[edit]Conclusion
[edit]Cloning of 2238 pEC2-uvrD
[edit]Purpose: Cloning of2238 pEC2-uvrD==
Date: 2014-12----
Notebook:0
Page:
Clone ID:2238
Experimenters: shanyi
Results
[edit]CLONING
[edit]PCR from MG1655
digest with Nco1 + XhoI, clone to 2015
Colony Screening
[edit]Date: 2014_7
Make miniprep, use Nco1 + XhoI to find positive clone