2240 pT7A3E-T4-uvsX
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Cloning of 2240 pT7A3E-T4-uvsX
[edit]Purpose: Cloning of2240 pT7A3E-T4-uvsX-==
Date: 2014-9----
Notebook:0
Page:
Clone ID:2240
Experimenters: shanyi
Results
[edit]CLONING
[edit]PCR from T4 phage Genomic DNA
Fragment 1
T4-uvsX-Nde1-F tcgaacatatgtctgatttaaaatctcgtttg
T4-uvsX-R1 gtgtttcgtatgtatggttaatagc
Fragment 2
Cloning Method
gene synthesis
Join two fragments
T4-uvsX-Nde1-F tcgaacatatgtctgatttaaaatctcgtttg
T4-uvsX-Xho1-R (gatatggaagaatttaatgaa ctcgag tagca) tgctactcgagttcattaaattcttccatatc
digest with Nde1 + Xho1
Colony Screening
[edit]Date: 2014_8
Make miniprep, use Nde1 + Xho1 to find positive clone