2244-pT7A3E-pelB-Lambda-Exo
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Cloning of 2244-pT7A3E-pelB-Lambda-Exo
[edit]Purpose: Cloning of 2244-pT7A3E-pelB-Lambda-Exo
Date: 2014-7-01---2014-7-02
Notebook:0
Page:
Clone ID:2244
Experimenters: LIANGHUO
Results
[edit]cloning
[edit]PCR from lambda DNA or clone 1026 pET28b-LambdaExo
1 ul of 10 uM pelB-Xba1-F
tagcTCTAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATAATGAAATACCTGCTGCCGACCGCTGCtGCTG
1 ul of 1 uM pelB-lambdaExo-F
CTGCTGCCGACCGCTGCtGCTGGTCTgCTGCTCCTCGCTGCCCAGCCGGCGATGGCCcatatgacaccggacattatcctgcag
1 ul of 10 uM LambdaExo-Xho1-R2
(gtatttggggagcaatggcga ctcgagcgatct)
agatcgctcgagtcgccattgctccccaaatac
2244 DIGESTED
[edit]
Colony Screening
[edit]Date: 2014-7-01
Make miniprep and digest with XbaI + XhoI
SELECT CLONE6 FOR SEQUENCING
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Result intepretation/Summary
[edit]Conclusion
[edit]