2245s 2246 2247 2248 2264 2051 2052 2055 2064 2068 2070

Protein Expression

2014/7/24
SHANYI

Lane	Name	Protein Name	Clone ID	Host	Induction	Purification	pI/Mw	express	sample
1	pT7A3E-OmpA-Lambda-Exo		2245	5 ml, Nico21(DE3)plysN	0.2 mM IPTG  RT O/N	Ni beads	6.13/29.2		media
2	pT7A3E-Lambda-Xis		2246	5 ml, Nico21(DE3)plysN	0.2 mM IPTG RT O/N	Ni beads	10.69/10.3	yes
3	pT7A3E-Int		2247	5 ml, Nico21(DE3)plysN	0.2 mM IPTG RT O/N	Ni beads	9.4/42
4	pT7A3E-UDG		2248	5 ml, Nico21(DE3)plysN	0.2 mM IPTG RT O/N	Ni beads	5.93/28.9
5	pT7A3E-Gst-Pol-1		2264	5 ml, Nico21(DE3)plysN	0.2mM IPTG RT O/N	Ni beads	6.12/69.3	yes
6	pT7A3E-Mxe-gyrA-ChiBD-6H		2051	5 ml, Nico21(DE3)plysN	0.2mM IPTG RT O/N	Ni beads	5.98/30.3
7	PageRuler Plus Prestained protein ladder
8	pT7A3E-Mxe-gyrA-CeBD-6H		2052	5 ml, Nico21(DE3)plysN	0.2mM IPTG RT O/N	Ni beads	5.6/42.3
9	pT7A3E-NpuDnaE-ChiBD-6H		2055	5 ml, Nico21(DE3)plysN	0.2mM IPTG RT O/N	Ni beads	5.24/19.8
10	pT7A3E-NpuDnaE-C1A-ChiBD-6H		2064	5 ml, Nico21(DE3)plysN	0.2mM IPTG RT O/N	Ni beads	5.24/19.8
11	pT7A3-6H-CBD-N-NpuDnaE-C1A		2068	5 ml, Nico21(DE3)plysN	0.2mM IPTG RT O/N	Ni beads	5.37/20.2
12	pT7A3E-NpuDnaE-C1A-CeBD-6H		2070	5 ml, Nico21(DE3)plysN	0.2mM IPTG RT O/N	Ni beads	4.78/33.6	yes