2290 2294 2360 2361 2379 2382 2385 1995 2038 1708

Protein Expression

2014/12/8

Lane	Name	Protein Name	Clone ID	Host	Induction	SAMPLE	pI/Mw	express
1	pT7A3-T7-DNA-helicase	T7-DNA-helicase	2290	15 ml, Nico21(DE3)plysN	0.2mM IPTG RT O/N	s	5.66/64.1	no
2			2290	15 ml, Nico21(DE3)plysN	0.2mM IPTG RT O/N	p		yes
3	pET28b-T7-DNA-helicase	T7-DNA-helicase	2294	15 ml, Nico21(DE3)plysN	0.2mM IPTG RT O/N	s	5.66/64.1	no
4			2294	15 ml, Nico21(DE3)plysN	0.2mM IPTG RT O/N	p		yes
5	pET30b-T4-uvsY	-T4-uvsY	2360	15 ml, Nico21(DE3)plysN	0.2mM IPTG RT O/N	s	17.5	no
6			2360	15 ml, Nico21(DE3)plysN	0.2mM IPTG RT O/N	p		no
7	pEB3-T4-uvsY	T4-uvsY	2361	15 ml, Nico21(DE3)plysN	0.2mM IPTG RT O/N	s		no
8			2361	15 ml, Nico21(DE3)plysN	0.2mM IPTG RT O/N	p		no
9	pageRuler Plus Prestained protein ladder
10	pT7A3-Cre-(Tev)-Tat-NLS-iFLP-6H	Cre-(Tev)-Tat-NLS-iFLP	2379	15 ml, Nico21(DE3)plysN	0.2mM IPTG RT O/N	S	9.63 / 93.1
11				15 ml, Nico21(DE3)plysN	0.2mM IPTG RT O/N	p		yes
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Lane	Name	Protein Name	Clone ID	Host	Induction	SAMPLE	pI/Mw	express
1	pT7A3-6XHis-SMT3-(Tev)	SMT3	2382	15 ml, Nico21(DE3)plysN	0.2mM IPTG RT O/N	s	5.75 / 17.8	yes
2			2382	15 ml, Nico21(DE3)plysN	0.2mM IPTG RT O/N	p		yes
3	pL6H-TRX-Tat-NLS-iFlp-6H	TRX-Tat-NLS-iFlp	2385	15 ml, Nico21(DE3)plysN	0.2mM IPTG RT O/N	s	9.32 / 66.1	no
4			2385	15 ml, Nico21(DE3)plysN	0.2mM IPTG RT O/N	p		no
5	pageRuler Plus Prestained protein ladder
6		kfu	1995	15 ml, Nico21(DE3)plysN	0.2mM IPTG RT O/N	s
7			1995	15 ml, Nico21(DE3)plysN	0.2mM IPTG RT O/N	p
8		Taq	2038	15 ml, Nico21(DE3)plysN	0.2mM IPTG RT O/N	s
9				15 ml, Nico21(DE3)plysN	0.2mM IPTG RT O/N	p
10	p	Cre	1708	15 ml, Nico21(DE3)plysN	0.2mM IPTG RT O/N	S
11				15 ml, Nico21(DE3)plysN	0.2mM IPTG RT O/N	p
12