2298 pEC2-T7-Endo1
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Cloning of 2298 pEC2-T7-Endo1
[edit]Purpose: Cloning of2298 pEC2-T7-Endo1==
Date: 2014-7--2014/8/1--
Notebook:0
Page:
Clone ID:2298
Experimenters: shanyi
Results
[edit]CLONING
[edit]PCR from 1225 pET28b-T7-Endo1
T7-endo1-Xba1-F tacta tctaga catatgg caggttacgg cgctaaag
T7-endo1-HindIII-R (taaaaaggaa aggaggaaag aaa ctcgag GGT ACC CACCACCACCACCACCACTGA aagctt tagca) tgctaaagcttTCAGTGGTGGTGGTGGTGGTGGGTACCctcgagtttctttcctcctttccttttta
2279pcr
digest with Xba1 + HindIII, clone to 2015 pEC2
Colony Screening
[edit]Date: 2014_8_1
Make miniprep, use Xba1 + HindIII to find positive clone
