2303 pEC2-Dnase1
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Cloning of 2303 pEC2-Dnase1
[edit]Purpose: Cloning of2303 pEC2-Dnase1==
Date: 2014-8----
Notebook:0
Page:
Clone ID:2303
Experimenters: shanyi
Results
[edit]CLONING
[edit]PCR DNase 1 from 2012-2 using
DNAse1-Nde1-F atcgac cat atg ctg aagatcgcagctttcaacatc
DNAse1-22 (5/3/2011)
(CACTAAGGTACCCTCGAGAATCC)
GGATTCTCGAGGGTACCTTAGTG
clone into 2015 using Nde1 +Xho1
Colony Screening
[edit]Date: 2014_7
Make miniprep, use Nde1 +Xho1 to find positive clone
Result intepretation/Summary
[edit]Conclusion
[edit]