2353 pFastbac-GP64-Flp-Flpe-WPRE
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Cloning of 2353 pFastbac-GP64-Flp-Flpe-WPRE
[edit]Purpose: Cloning of2353 pFastbac-GP64-Flp-Flpe-WPRE==
Date: 2014-9----
Notebook:0
Page:
Clone ID:2353
Experimenters: shanyi
[edit]
Results
[edit]CLONING
[edit]subclone from 1981 pCMV5-Tat-Flp-PDGFR to 2354 pFastbac-GP64-Flp-WPRE using Nhe1 + Kpn1
Colony Screening
[edit]Date: 2014_8
Make miniprep, use Xho1 + Kpn1 to find positive clone