2356 pFastbac-GP64-iCre-iCre

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Cloning of 2356 pFastbac-GP64-iCre-iCre

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Purpose: Cloning of2356 pFastbac-GP64-iCre-iCre==

Date: 2014-9----

Notebook:0

Page:

Clone ID:2356

Experimenters:  shanyi


Results

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CLONING

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subclone from 1972 pCMV5-Tat-Cre-PDGFR to 2352 pFastbac-GP64-Cre-Flpe-WPRE using Nhe1 + Kpn1


Colony Screening

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Date: 2014_8

Make miniprep, use Xho1 + Kpn1 to find positive clone,will get two bands


digest 2356-1(11/17)with NheI to comfirm,will get one band



Result intepretation/Summary

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Conclusion

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