2374 pFastbac-oriP
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Cloning of 2374 pFastbac-oriP
[edit]Purpose: Cloning of2374 pFastbac-oriP-==
Date: 2014-9----
Notebook:0
Page:
Clone ID:2374
Experimenters: shanyi
Results
[edit]CLONING
[edit]PCR from 371 pCEP4 E02S CK2M EN2L
2374-oriP-ENBA-Xho1-R
(CTTGTCCTTTTCCTGCTGGTTG CTCgag tactaa)
ttagtactcGAGCAACCAGCAGGAAAAGGACAAG
2374-oriP-ENBA-Kpn1-F
tagcacGGTACCTGTAATCCCTTCAGTTGGTTG
Digest with Kpn1-Xho1
Colony Screening
[edit]Date: 2014_9
Make miniprep, use Kpn1-Xho1 to find positive clone
