2401 pET30b-scRAD51
Cloning of 2401 pET30b-scRAD51
[edit]Purpose: Cloning of2401 pET30b-scRAD51==
Date: 2015_1
Notebook:0
Page:
Clone ID:2401
Experimenters: shanyi
Results
[edit]CLONING
[edit]PCR from 2396
1.
2401-scRAD51-Nde1-F
tactaacatatggcgtttttaagctattttg
2401-RAD51-R
cttctagcatgtgaggccccag
2.
2401-scRAD51-F
ctggggcctcacatgctagaag
2401-scRAD51-Xho1-R
(ctgcatgcacgcaagcctact ctcgag tatcac)
gtgatactcgagagtaggcttgcgtgcatgcag
3.
2401-scRAD51-Nde1-F
tactaacatatggcgtttttaagctattttg
2401-scRAD51-Xho1-R
(ctgcatgcacgcaagcctact ctcgag tatcac)
gtgatactcgagagtaggcttgcgtgcatgcag
digest with Nde1 + Xho1
Colony Screening
[edit]Date: 2015_1
Make miniprep, use Nde1 + Xho1 to find positive clone
CLONING
[edit]PCR from 2396
2401-scRAD51-Nde1-F tagataccATG ggt TCTCAAGTTCAAGAACAACATATATC
2401-scRAD51-Xho1-R (GACCCCAGAGAAGAAGACGAG ctcgag tagcac) gtgctactcgagCTCGTCTTCTTCTCTGGGGTC
digest with Nco1 + Xho1
Colony Screening
[edit]Date: 2015_1
Make miniprep, use Nco1 + Xho1 to find positive clone
