2403 pEC-35S-Promotor
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Cloning of 2403 pEC-35S-Promotor
[edit]Purpose: Cloning of2403 pEC-35S-Promotor==
Date: 2015_1
Notebook:0
Page:
Clone ID:2403
Experimenters: shanyi
Results
[edit]CLONING
[edit]PCR from 1153 pCAMBIA-2301
2403-35S-Promotor-EcoR1-F
tatac gaattc AACATGGTGGAGCACGACACTCTC
2403-35S-Promotor-Nde1-R
tgctacataTGTTCTCTCCAAATGAAATGAAC
(GTTCATTTCATTTGGAGAGAACAtatg tagca)
digest with Nde1 + EcoR1
Colony Screening
[edit]Date: 2015_1
Make miniprep, use Nde1 + EcoR1 to find positive clone
