2404 pET28b-6H-TRX
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Cloning of 2404 pET28b-6H-TRX
[edit]Purpose: Cloning of2404 pET28b-6H-TRX==
Date: 2015_1
Notebook:0
Page:
Clone ID:2404
Experimenters: shanyi
Results
[edit]CLONING
[edit]PCR from 1737 using
2404-6H-TRX-Nco1-F
Tagcaa ccatgggcagcagccatcatcatcatcatcacagcagc AGCGATAAAATTATTCACCTG
TRX-Nde1-R
(AGTTCCTCGACGCTAACCTGGCCggggatgacgatgacaagGAAAACCTTTACTTCCAGcatatg cgatca )
tgatcgcatatgCTGGAAGTAAAGGTTTTCcttgtcatcgtcatccccGGCCAGGTTAGCGTCGAGGAACT
digest with Nde1 + Nco1
Colony Screening
[edit]Date: 2015_1
Make miniprep, use Nde1 + Nco1 to find positive clone
