2406 pEC-35S-Miraculin
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Cloning of 406 pEC-35S-Miraculin</span
[edit]Purpose: Cloning of 406 pEC-35S-Miraculin
Date: 2015-3-13
Notebook:
Page:
Clone ID: 2412
Experimenters: Paul & Xu Xin
Results
[edit]cloning
[edit]Date: 3/14/2015
Digest plasmid DNA with Nde1 + EcoR1
combine insert from 2403 and digested vector 2402, purify, ligate, transform DH5alpha
screeing 6 colony from transformation plate, mini-pre, digestion, screening. All the 6 colonies have the product. make the glycerol stock as 2406 use the first digstion plasmid. For the plasmid stock 2406 with the plasmid#1; and mix all the other plasmids make 2406 mix
Colony Screening
[edit]Date: