2422 pEC-35S-Miraculin-Term

Cloning of 2422 pEC-35S-Miraculin-Term

Purpose: Cloning of 2422 pEC-35S-Miraculin-Term

Date:  2015-3-21

Notebook: 

Page: 

Clone ID: 2422

Experimenters: Paul

Results

subcloning from  2406  pEC-35S-Miraculin to  2420 pEC-Miraculin-Term  using EcoR1 + Kpn1

Date: 3/21/15

1: 2420 pEC-Miraculin-Term, Kpn1 + Nco1

2: 2406  pEC-35S-Miraculin, EcoR1 + Kpn1

3: 2420 pEC-Miraculin-Term, EcoR1 + Kpn1

cut out insert from 3, and vector 2, add QG, purify, ligate and transform DH5alpha.

Colony Screening

Date:  2015