2441-PDB.His.GST-MeCP2-MBD

Cloning of 2441-PDB.His.GST-MeCP2-MBD

Purpose: Cloning of human recombinant; methyl binding domain aa 77-166 into vector 433 PDB.His.GST

Date: 2015-May

Clone ID:2441

Experimenter: Xin

Results:

Gene systhese: Human recombinant; methyl binding domain aa 77-166: 300bp

The vector 433 pDB.His.GST was digested by NdeI/XhoI

Digest the PCR product, ligation, transformation.

There wasn't any colony growing on the plate.

Repeat again, same result as last one, no colony growing on the plate.

Redo again, there was one colony growing on the plate. Mini preparation, digestion.

There was a faint band around 300bp, but hard to see. Redigest again,

Still hard to see. Maybe it wasn't a positive plasmid. Or need other method to verify it is a positive plasmid.