2459-pET30b-Tat-NLS-Cas9

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Subcloning from 2332&1291

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Digest by Kpn1+Xho1


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digested by Nde1 and Xho1

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After ligation and transformation, pick up colonies from the plate and do screening,digested by Nde1 and Xho1


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Sequencing and make glycerol Stock

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