2461 2462 2467 Protein expression screening

Lane	Name	Protein Name	Clone ID	Host	Induction	Purification	pI/Mw
1	pET28b-6H-Tat-NLS-Cas9		2461	5 ml, Nico21(DE3)plysN	0.2 mM IPTG  RT o/n	Ni beads	9.36 / 164777.95
2	pT7A3E-Tn5-ChiBD-6H		2462	5 ml, Nico21(DE3)plysN	0.2 mM IPTG  RT o/n	Ni Beads	8.41 / 83.8
3	pET30-30GFP-Cas9		2467	5 ml, Nico21(DE3)plysN	0.2 mM IPTG RT o/n	Ni beads	7.66 / 189.5
4	Marker

Weak band in 2462 pT7A3E-Tn5-ChiBD-6H(83.8kDa)

no expression in 2467 pET30-30GFP-Cas9 (189.5 kDa)and 2461 pET28b-6H-Tat-NLS-Cas9(166.9 kDa)

Lane	Name	Protein Name	Clone ID	Host	Induction	Purification	pI/Mw
1	pET28b-6H-Tat-NLS-Cas9		2461	5 ml, Nico21(DE3)plysN	0.2 mM IPTG  RT o/n	Ni beads	9.36 / 164777.95
2	pET28b-6H-Tat-NLS-Cas9		2461	5 ml, Nico21(DE3)plysN	0.2 mM IPTG  RT o/n	Ni beads	9.38 / 166.9
3	Marker
4	pT7A3E-Tn5-ChiBD-6H		2462	5 ml, Nico21(DE3)plysN	0.2 mM IPTG RT o/n	Ni beads	8.41 / 83.8
5	pT7A3E-Tn5-ChiBD-6H		2462	5 ml, Nico21(DE3)plysN	0.2 mM IPTG RT o/n	Ni beads	8.41 / 83.8