2615

2615 pCold-MBP-(tev)-GST-(3c)-Ac-LysargiNase

expression screening, 9/27/19

Lane	CLone ID	Name	Induction	Fraction	Expression
1	2615	pCold-MBP-(tev)-GST-(3c)-Ac-LysargiNase	25 oC , 4h	soluble
2	2615		37 oC 4 h	soluble	Y
3	2616	pET28b-hFth-CYFRA 21-1-avitag-6H	25 oC , 4h	soluble	Y
4	2616		37 oC 4 h	soluble	Y
5	2621	pET29a-NSP4-iFlp	25 oC , 4h	soluble
6	2621		37 oC 4 h	soluble
7	2615	pCold-MBP-(tev)-GST-(3c)-Ac-LysargiNase	25 oC , 4h	IB	Y
8	2615		37 oC 4 h	IB	Y
9	2616	pET28b-hFth-CYFRA 21-1-avitag-6H	25 oC , 4h	IB	Y
10	2616		37 oC 4 h	IB	Y
11	2621	pET29a-NSP4-iFlp	25 oC , 4h	IB
M		1250, 130, 100, 70, 55, 35, 25, 15, 10

Lane	ID	Name	Induction	Sample	Expression
1	2555		5052 o/n 37 oCm 1 mM 3 hours 37 oC
2	2556		5052 o/n 37 oCm 1 mM 3 hours 37 oC
3	2557		5052 o/n 37 oCm 1 mM 3 hours 37 oC
4	2558		5052 o/n 37 oCm 1 mM 3 hours 37 oC
5	2559		5052 o/n 37 oCm 1 mM 3 hours 37 oC
6	2560		5052 o/n 37 oCm 1 mM 3 hours 37 oC
7	2561		5052 o/n 37 oCm 1 mM 3 hours 37 oC
8	1562		5052 o/n 37 oCm 1 mM 3 hours 37 oC
9	2563		5052 o/n 37 oCm 1 mM 3 hours 37 oC
10	2564		5052 o/n 37 oCm 1 mM 3 hours 37 oC, cell lysed
11	971		5052 o/n 37 oCm 1 mM 3 hours 37 oC, cell lysed
M
12	2621	pET29a-NSP4-iFlp, 9.76 / 52.8	5052 o/n 37 oCm 1 mM 3 hours 18 oC	S
13	2615	pCold-MBP-(tev)-GST-(3c)-Ac-LysargiNase, 5.23 / 110.7	5052 o/n 37 oCm 1 mM 3 hours 18 oC	S
14	2615	pCold-MBP-(tev)-GST-(3c)-Ac-LysargiNase	5052 o/n 37 oCm 1 mM 3 hours 18 oC	IB