2752
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2752 with ccdb , cut with BamH1 + PacI and reliate, transform with ccdb survival, no colony grow on LB/amp,
2752 cut with BamH1 + PacI, ligate to BamH1-PacI fragment from145
colonies turn green under bluelight,
plasmi digestion BamH1+ PacI or EcoRV + PvuII
multiple bands, could be incomplete digestion
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[edit]2752 sfGFP in pMiniT2, subcloned from 145 using BamH1-PacI, Plasmid digest with EcoRV-PvuII
