2952
Jump to navigation
Jump to search
PCR amp from 2889 using 2952-F + 2952 R, digest PCR product with Nde1 + Mlu1
digest 2952 with Nde1 + Mlu1, cut out 1991 small band
ligate and transform DH10g
PCR amp from 2889 using 2952-F + 2952 R, digest PCR product with Nde1 + Mlu1
digest 2952 with Nde1 + Mlu1, cut out 1991 small band
ligate and transform DH10g