3027 pCEH0-mIgG2b-HC

3020-Xba1-Nhe1-F-> it is the same as IgG2b
        Xba1            Nhe1
atcatc TCTAGA ttagaatc GCT AGC AGC GCT AAA ACG ACA CCC CCA TCT GTC TAT C
                        A   S   S   A   K   T   T   P   P   S   V   Y 

3027-Not1-Age1-R
2b atcatcACCGGTGCGGCCGCTCATTTACCCGGAGACCGGGAG (CTCCCGGTCTCCGGGTAAATGA GCGGCCGC ACCGGT gatgat)
   S  R  S  P  G  K  -

Subclone of 3027:

Vector:3017pCEH0

Insert:3027pMiniT2

restriction enzymes:MheI+NotI

Time:30 min

Result:So we cut out the band and did subclone

3021:1-4 restriction enzymes:BssHII+XbaI

3027:5-8 restrcition enzymes:NotI+NheI

Buffer:Fastdigest

Time:16min

Result:seems good, but the result of sequencing is not,the product were actually are undigested vectors,3016pCEL0,3017pCEH0. so we collected them for other using.