3132 pCEH0-HA-VH-mIgG2a

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Miniprep of 3168_3132_3161

3168:1-4 BsrGI+NheI

3132:5-8 BsrGI+NheI

3161:9-12 NdeI+XhoI

Buffer:Fastdigest

Time:30Min

Result:#7 seems good, so sent to do sequencing, the rest of them are suspicious