3152 pCEH0-Flag-VH-mIgG2a
Jump to navigation
Jump to search
Repeat subclone of 3152
Restriction enzymes:BsrGI+NheI
Buffer:NEB2
Time:30min
Result:Vector digest is good, but insert is not good.
Miniprep of 947_947pMiniT2_3131_3152
947:1-4 XbaI+NcoI-HF 1394 Cutsmart
947pMiniT2:5-8 ECoRI Cutsmart
3131:9-12 BssHII+XhoI 342 Cutsmart
3152:13-16 BsrGI+KpnI 371 NEB 2
Result: sent lane 2, lane 16 to do sequence
Date:4/2/2018
[edit]redo subclone of 3152
Vector:3163
Insert: 3009pMiniT
enzymes:BsrGI+NheI
Date:4/4/2018
[edit]miniprep digestion of 984_947_3152
- Name Enzymes Size:
1-4:984 XbaI+NheI 957bp
5 :982(973-1pMiniT) XbaI+NheI
6-9:947 XbaI+NcoI-HF 1344bp
10:947pMiniT XbaI+NcoI-HF
11:947(from PCR cloning) XbaI+NcoI-HF
12-15:3152 BsrGI+NheI 371bp
16: 3162(Vector) BsrGI+NheI
