3191 pT7A3-GST-Avitag

Vector digestion

Miniprep digestion of 2503_3122_3197_3191

#	lane	Enzymes	Size	Note
2503	1-4	Nde1+Xho1	951bp	#1,2 seems good
3122	5-8	Nde1+Xho1	1743bp	#5,6,8 seems good
3197	9-12	BamH1+Nco1	1 band	may not good
3191	13-16	BamH1+Nco1	722bp	#14,15,16 seems good

send clone #14 to sequencing, T7-R, 

sequencing result: no insert (empty vector--vector was not digested completely)QC method: digest with Xba1-Xho1: 818bp

digest with Xba1-EcoR1: only lineared vector optionally, sequencingdigest wit

Date:6/5/2018

Miniprep of 3191_984_995

#	Lane	Enzymes	Size	Note
3191	1-7	BamH1+Nco1	no bands	#5,#6 are good
984	8-11,12 is +control	Xba1+Nhe1	~986bp	#10 is goood
995	13-16	Nde1+Xho1		not good

Dat3:6/8/2018

Because cut by BamH1+Nco1 above on Date6/5/2018,

#5 and #6 has no bands on the gel, so did another miniprep digestion to confirm

Miniprep of 3191

Enzymes:Xba1+Xho1 & Xba1+EcoR1

Result intepretation

digest with Xba1-Xho1: 818bp

digest with Xba1-EcoR1: only lineared vector 

so it could be good