3226 pEPD-PHILOtop

Cloning

5/30/2018: Dissolve IDT gblock DNA in 10 ul H2O, take 1 ul for digestion with 0.5 ul SfiI in 15 ul vol,  50 oC for 2 hours. co-Purify SfiI-digested vector using spin column, elute with 20 ul H2O, ligate at RT for 30min with 1 ul T4 DNA ligase, transform 10G

6/2/2018: Miniprep digest NcoI + Xho1