3255 pCMV5-myc-rk
Jump to navigation
Jump to search
Cloning of 3255 pCMV5-myc-rk
[edit]Purpose: Cloning of 3255 pCMV5-myc-rk
Date: 08, 15, 2018
Notebook:
Page:
Clone ID: 3255
Experimenters: yijia
Results
[edit]Cloning
[edit]PCR from 3213 pANT7_cGST-MICA
1st PCR of 3255
template:
F1:3075 PCR from 3075 pCMV5-Myc-L-kappa
3255-EcoR1-F1 TGTCACGAATTCAgacatcgtgctgacacagag
F2:1414 PCR from 1414 pCDNA3.4-Rabbit-IgG Light Chain
3255-F2 gccggaacaaagctggaaatcaagGCTAGCGGTACCGATCCAGTTGCACCTACTG
F3:1414 PCR from 1414 pCDNA3.4-Rabbit-IgG Light Chain
3255-F3 CACCGCAGAACTCTGCAGATTGTAC
2nd PCR of 3255
Template:combine F1+F2+F3 3255-EcoR1-F1 TGTCACGAATTCAgacatcgtgctgacacagag
Vector digestion
Vector is 3075
Colony screen
[edit]
Miniprep of 3255
| # | Lane | Enzymes | Length | Buffer | Note |
| 3255 | 5-8(#1-4) | EcoR1+Not1 | 671bp | Fastdigest 30min | #4 to do sequence |
| vector control | 3075 | EcoR1+Not1 | 685bp | Fastdigest 30min |
