3274 pT7A3-MBP-GST-Avitag-BirA

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Cloning of 3274 pT7A3-MBP-GST-Avitag-BirA

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Purpose: Cloning of 3274 pT7A3-MBP-GST-Avitag-BirA

Date: 08, 22, 2018

Notebook: 008840

Page:

Clone ID: 3274

Experimenters: yijia

Results

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Cloning

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3274 PCR

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Vector digestion

vector is 3249

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 Colony screen

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Miniprep digestion of 3274

Xba1+Nco1 1245bp Fastdigest

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3274 Miniprep digestion

Lane 1,2,3,4, are #1,#2,#3,#8

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10/9/2018

3274 vector digestion with Nde1, Xho1, Nde1+ Xho1, --> multiple bands

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Result intepretation/Summary

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Conclusion

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