332-pLenti-RFP-LoxP-GFP
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Cloning of pLenti-RFP-LoxP-GFP (332)
Purpose: to clone pLenti-RFP-LoxP-GFP by PCR
Date: 2011-9-20 to 2011-9-
Notebook: Xiaojing Wang
Page: 004095---004096
Clone ID: 332
Experimenters: Xiaojing Wang
Results
TagGFP was amplified by gene synthesis.
The right band shows PCR product of LoxP-TagGFP, TagGFP as template
LoxP-TagGFP-EcoR1-F C4 atcga gaattc ATAACTTCGTATA ATG TAT GC T ATA CGA AGT TAT AGC GGG GGC GAG GAG CTG
TagGFP-R1 XhoI (CACGGCATGGACGAGCTGTACAGGTAA ctcgag atcga) tcgatctcgagTTACCTGTACAGCTCGTCCATGCCGTG
Digest result:
digest PCR product and pLenti-mKate2 vector with 0.5ul EcoR1+ 0.8ul Xho1 at 37 for 1 hour , run gel
gel purify, ligate, transform STBL3 (Amp)
Colony Screening