367 368 369 370 377 378 sso7d Taq Pfu
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Cloning of sso7d fusion to Taq and Pfu (367, 368, 369, 370, 377, 378)
[edit]Purpose: make sso7d fusion to Taq and Pfu.
Date: 2011-9-2 to 2011-9-
Notebook:
Page:
Clone ID:
Experimenters: Paul
[edit]
Results
[edit]PCR
[edit]'
'
[edit]
Restriction Digestion Screening of colonies
[edit]
| Sample # |
Sample |
Enzyme |
DNA conc |
260/280 |
| 1 |
367-1, pET30b-Pfu |
Nde1-KpnI |
||
| 2 |
367-2 |
Nde1-KpnI |
||
| 3 |
367-3 |
Nde1-KpnI |
||
| 4 |
367-4 |
Nde1-KpnI |
||
| 5 |
367-5 |
Nde1-KpnI |
||
| 6 |
368-1, pET30bsso7d-KX |
Kpn1+ Xho1 |
||
| 7 |
368-2 |
Kpn1+ Xho1 |
||
| 8 |
368-3 |
Kpn1+ Xho1 |
||
| 9 |
368-4 |
Kpn1+ Xho1 |
||
| 10 |
368-5 |
Kpn1+ Xho1 |
||
| 11 |
369-1, pET30b-sso7d-NK |
Nde1-KpnI |
||
| 12 |
369-2 |
Nde1-KpnI |
||
| 13 |
369-3 |
Nde1-KpnI |
||
| 14 |
369-4 |
Nde1-KpnI |
||
| 15 |
369-5 |
Nde1-KpnI |
||
| 16 |
pEt30b-Taq_d289 370-1 |
Kpn1+EcoR1 |
||
| 17 |
370-2 |
Kpn1+EcoR1 |
||
| 18 |
370-3 |
Kpn1+EcoR1 |
||
| 19 |
370-4 |
Kpn1+EcoR1 |
||
| 20 |
370-5 |
Kpn1+EcoR1 |
sequencing:
--Paul.xu 09:56, 6 September 2011 (EST)